過(guò)敏性紫癜兒童SOCS表達(dá)及與Th17-Treg細(xì)胞失衡關(guān)系研究.pdf

1、分類號(hào):R729 密級(jí)： UDC：610 學(xué)校代碼：11065 碩士學(xué)位論文 過(guò)敏性紫癜兒童 過(guò)敏性紫癜兒童 SOCS 表達(dá)及與 表達(dá)及與 Th17/Treg 細(xì)胞失衡關(guān)系 細(xì)胞失衡關(guān)系 研究 研究 訾廣芹 訾廣芹 指 導(dǎo) 教 師 常紅 副教授 學(xué)科專業(yè)名稱 兒科學(xué) 論文答辯日期 2015

2、 年 5 月 29 日 The expression of SOCS protein in peripheral blood mononuclear cell and the balance of Th17 /Treg in children with Henoch-Sch?nlein Abstract Objective We investigate the expression of SOCS in peripheral blood

3、 mononuclear cells and its correlation with Th17/Treg immune response in children with HSP, to explore the relationship between SOCS and the imbalance of Th17/Treg immunological pathogenesis of HSP. Methods Totally 32

4、children with Henoch-Schö nlein purpura were enrolled in the study. Another 20 healthy age-matched children were simultaneously taken as control. Protein levels of CD4+CD+25、CD4+IL-17A+ and mean fluorescence intens

5、ity (MFI) for phosphorylated-STAT3(pSTAT3) protein in CD4+T cells was detected by flow cytometric analysis. The relative expression level of SOCS1 mRNA、SOCS3 mRNA in peripheral blood mononuclear cells was detected by re

6、al-time fluorescent PCR. The protein level of IL-6 was assessed by ELISA. Results 1.The levels of IL-6 and MFI pSTAT3 protein in CD4+T cells of the HSP children were significantly higher than the control group(t=16.49，P

7、＜0.01；t=9.34，P＜0.01). 2.The levels of CD4+IL-17A+ in CD4+T cells of the HSP children were significantly higher than the control group (t=7.59,P＜0.05), but the levels of CD4+CD25+ in CD4+T cells of the HSP children were

8、lower than the control group(t=4.58,P＜0.01). 3. The mRNA expression of SOCS1、SOCS3 in CD4+ cells increased significantly during acute phase of HSP. 4. The mRNA expression of SOCS1、 SOCS3 showed a negative correlation wit

9、h expression of Th17/Treg of the CD4+T cells in HSP children (r=-0.328,P＜ 0.05;r=-0.413,P＜0.01）. Conclusions There were both IL-6/STAT3 existed in children with HSP and existence of imbalance of Th17/Treg which were the

10、 aberrant of Th17 and the insufficient of Treg. There were higher mRNA expression of SOCS1、SOCS3 in CD4+ cells increased significantly during acute phase of HSP and it was a negative correlation with expression of Th17/

11、Treg of the CD4+T cells in HSP. The insufficient of expression level of SOCS1 mRNA、SOCS3 mRNA might lead to abnormal of IL-6/STAT3, It might be associated with the imbalance of Th17/Treg in children with HSP. Postgradua