低氧及跑臺訓練對大鼠骨骼肌總蛋白和骨骼肌IGF-Ⅰ基因表達的影響.pdf

1、南京體育學院碩士學位論文低氧及跑臺訓練對大鼠骨骼肌總蛋白和骨骼肌1GFI基因表達的影響InfluenceofhypoxiaandtreadmilltrainingtotheconeentrationproteinofskeletalmuscleandGeneexpressionofskeletalmuscleIGFIinrats培養(yǎng)單位—垂盤叢盤生目蟲L—————一學科專業(yè)—垂薹生生鹽立匕fL——————一研究方向女!I堡墮控焦垂盈絲疸

2、羞生送復碩士生姓名——j鹽j奠生L—————一指導教師——蘭—!墮——————一南京體育學院碩Ij學位論文Influenceofhypoxiaandtreadmi11trainingtotheconcentrationproteinofskeletalmuscleandGeneexpressionofskeletalmuscleIGFIinratsAbstractStudypurpose：(1)Influenceofthehypoxia

3、andtreadmilltrainingtototalproteinoftherat’Sskeletalmuscle(2)tounderstandthechangeruleoftherat’SskeletalmuscleIGF—IgeneexpressionintheprocessofhypoxiaandtreadmilltrainingStudymethods：Randomdivide60maleSDratswhichwerescre

4、enedinto6groups：NC，HC，NEL，HEL，NEH，HEH，tenratseachgroupIntensityis152m／minand268m／minrespectively，inclinationis10degree(NEL)、(NEH)、(HEL)and(HEH)fourgroupsaretrainingundernormaloxygen，thetrainingtimeis40minperday，sixdaysaw

5、eekHELandHEHgroupsarelivingunderlowoxygenTheoxygenthicknessiscontrolledto141％inlowoxygenenvironmentwhichisequalinelevation3000mLowoxygengroupratsliveinlowoxygencanvasfrom8pmeachdayto8amnextdayFourgroupofexperimentalratst

6、ake2weeklongadaptabletrainingand5week—longformaltrainingTakingrats’thighsuralmusclewithin24hoursafterthelasttimetraining，theninterceptthesamepartofthemiddleofmuscle。UsingBradfordmethodtodetermineproteindensityandtestinga

7、bsorbencycancalculatethecontentofproteinUsingWesternBlottingmethodtodetermineIGF—IcontentofmusclethenextractmRNAUsingRT—PCRtoexamineIGF—ImRNAexpressionofeachgroupConclusion：Afterfive—weekhypoxiaexercise，thesportinggroupr

8、ats’bodyweightobviouslydroppedamongwhichtheHEH’SwasthelowestItpromptsthatthelowoxygenmovementhastheinhibitoryactiontotherat’SbodyweightTheNELgroup，NEHgroupratsskeletalmuscleIGFIandthetotalproteinobviouslyelevatedItiScons

9、istenttothereportthatmovementcanpromotetheskeletalmuscleIGF—IsecretionandincreasetheskeletonproteinsynthesisHELgroupelevatedobviouslycomparedwithHCgroup，HEHgrouphadnochangesItpromptsthatthedifferentmovementintensityhasdi

10、fferenteffecttats’skeletalmuscleIGF—IandthetotalproteinNELgroupcomparedwiththecorrespondinggroupelevatedobviouslyonIGF—ImRNATheHELgroupelevatedslightlyandHEHgroupdroppedsignificantlyItpromptsthatitexistsdifferenceofIGF—’